The Team
  • Researchers

    Claude ANTONY

    Adam BEN SHEM

    Philippe DUMAS

    Gabor PAPAI

    Patrick SCHULTZ

  • Post-Doctoral Fellows

    Olga KOLESNIKOVA

    Grigory SHAROV

  • Engineers & Technicians

    Pierre ANTONY

    Corinne CRUCIFIX

    Christine RUHLMANN

    Danièle SPEHNER

Integrated structural Biology

Architecture of nucleoprotein systems by 3-D Electron Microscopy      

Our team deciphers the three-dimensional organization of macromolecular complexes by using high resolution cryo- Electron Microscopy (EM) to visualize single molecules in their native state and image analysis to extract signal from noise. We study how basal transcription factors interact with the promoter of protein-encoding genes and analyze the transmission of activation signals mediated by cis-acting transcription factors towards the basal transcription machinery in order to trigger increased transcription. The study of different intermediate states reveals snapshots of dynamic processes that highlight the conformational changes of these nanomachines during transcription initiation. These approaches were applied to several molecular systems including the SAGA and TFIID coactivators, but also to other systems such as TFIIH, TFIIE, RNA Polymerase I, DNA Topoisomerase II or HIV integrase, in collaboration mainly with IGBMC team leaders. As part of an integrated vision of cellular structural biology we analyze the organization of transcription within the nuclei of rod photoreceptors. The biological system used is a mouse model of a human disease: the type 7 Spinocerebellar Ataxia. Chromatin organization, histone modification and transcriptional activity were correlated in this context.

Imprimer Envoyer

Université de Strasbourg
INSERM
CNRS

IGBMC - CNRS UMR 7104 - Inserm U 964
1 rue Laurent Fries / BP 10142 / 67404 Illkirch CEDEX / France Tél +33 (0)3 88 65 32 00 / Fax +33 (0)3 88 65 32 01 / directeur.igbmc@igbmc.fr