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PluriCell East
PluriCell East
With over 30 years of experience in cell culture, we provide a wide range of services from media manufacturing to cell banking.
We have a collection of over 2400 cell lines. These include normal cell lines, tumor cell lines, cell lines from patients with genetic diseases as well as cell lines from different species (human, rodent and other mammals, zebrafish, insects, …).
For over 10 years, we have been developing expertise in the field of human induced pluripotent stem cells (hiPSC) and mouse embryonic stem cells (mESC).
These services are intended for the entire scientific community, national and international, public and private.
The PluriCell East platform provides expertise, technological and scientific support to help users. To this end, it:
- Performs human and animal cell lines culture, both normal and patient-derived
- Performs large-scale cell suspension culture
- Performs mouse embryonic stem cells (mESC) and human induced pluripotent stem cells (hiPSC) culture
- Prepares mouse embryonic fibroblasts (MEF) and inactivated MEF with mitomycin C (Feeders) for mESC culture
- Produces LIF (Leukemia Inhibitory Factor)
- Prepares customized culture media
- Performs quality controls
- Thawing tests
- Mycoplasma detection tests
- Characterization of induced pluripotent stem cells (hiPSC)
- Chromosome counting
- Performs optimizations of culture conditions and testing of new products (tests of materials, media, serum, culture methods etc.)
- Provides technical support to researchers for their projects
- Trains new users in hiPSC culture
- Provides a L2 laboratory for hiPSC manipulation
- Provides a L2 laboratory for virus manipulation
120 m² cell culture laboratories (L1 et L2)
9 Biological Safety Cabinets HERASAFE KS12 et KS15
12 Incubators CO2 Panasonic
3 inverted Zeiss Microscopes
2 observation system Lynx Stereo Vision Engineering for hiPSC culture
1 Analyseur-Coulter Beckman Vi-Cell XR
1 room at 37°C for large scale suspension cells culture
2 Laboratories L2 dedicated to hiPSC culture:
- 7 Biological Safety Cabinets HERASAFE KS12 et KS15
- 6 incubators CO2 Panasonic
- 1 incubator tri-gas for culture in hypoxia
- 4 Leica observation systems
- 2 Lynx observation system Stereo Vision Engineering
- 1 inverted Microscopes whose 1 fluorescence microscope with camera
For any request to the PluriCell East Platform, please contact us at :
pluricell-east@igbmc.fr
The PluriCell East platform is involved in several regional, national and European networks :
- CoRTeCS (https://cortecs.unistra.fr/)
- CorEuStem (https://coreustem.eu/)
- Cancéropole Grand Est (https://www.canceropole-est.org/)
- GDR organoïdes (https://gdr-organoides.cnrs.fr/)
Publications
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2022
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Cell cycle gene regulation dynamics revealed by RNA velocity and deep-learning
- Andrea Riba
- Attila Oravecz
- Matej Durik
- Sara Jiménez
- Violaine Alunni
- Marie Cerciat
- Matthieu Jung
- Céline Keime
- William M Keyes
- Nacho Molina
Nature Communications ; Volume: 13 ; Page: 2865
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2021
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Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network
- Valérie Schreiber
- Reuben Mercier
- Sara Jiménez
- Tao Ye
- Emmanuel García-Sánchez
- Annabelle Klein
- Aline Meunier
- Sabitri Ghimire
- Catherine Birck
- Bernard Jost
- Kristian Honnens de Lichtenberg
- Christian Honoré
- Palle Serup
- Gérard Gradwohl
Molecular metabolism ; Volume: 53 ; Page: 101313
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