Cells and tissues in 3D with the new FIB-SEM microscope
July 9, 2017
A new Focused Ion Beam-Scanning Electron Microscopy (FIB-SEM) was delivered to CBI. This € 800,000 FRISBI and abEx-funded microscope aims to observe cellular or tissue samples in three dimensions with very high precision. The riginal technique is based on the use of two beams: a alium beam (FIB) which cuts extremely thin lices (only 5 nm) of the sample, coupled to an electron beam (SEM) Intracellular organization.
By repeating these two actions many times, the 3-D information is accessed by tomography. This is one of the most accurate methods for observing and quantifying subcellular structures at the scale of an entire cell. Thanks to the development of algorithms for the analysis of the results, Patrick Schultz's team succeeded in quantifying several parameters of the cell nucleus such as the volume occupied by the nucleus, the chromatin or the nucleolus or to count the exact number of pores. Commissioned in January,this material is destined to be used by the broader scientific community but requires the accompaniment of an expert.
For more information, please contact Patrick Schultz.