Post Doc - Equipe N. Ghyselink - Manuel Mark
Reference : IGBMC / Post doc N.V.
Publication de l'offre : 9 juillet 2018
Uncover the biological functions and the molecular mechanism by which the nuclear receptor coregulators affect spermatogenesis
Nuclear receptors (NR) are ligand-dependent transcription factors that activate or repress transcription of genes critical to biological processes such as development, reproduction, and homeostasis. In the absence of ligand, NR actively repress transcription via interactions with the corepressors (NCoR). Upon binding of the ligand to the NR, corepressors dissociate away from the DNA-bound receptor that now recruit coactivators (NCoA). These interacting coregulators are of great importance because coregulators have the potential to give a better insight into functioning of NR signaling pathways.
Our experimental model, the seminiferous epithelium, composed of somatic cells (Sertoli cells) and of germ cells, represents an excellent paradigm to investigate the NR signaling pathways, because (i) more than 20 distinct NR are expressed in this model system, the function and/or the molecular mechanism of action of which remain to be investigated for a lot of them, and (ii) this model integrates the problematics of stem cell renewal, cell proliferation, switching from mitotic to meiotic cell division, programmed cell death and paracrine signaling, all of which are potential targets for pathological deregulations.
Through focusing on Sertoli cells, the project proposes to characterize yet unidentified signaling pathways acting to control these pathophysiological processes, collectively named spermatogenesis. To this aim, the project proposes to uncover the biological functions and the molecular mechanisms of NCoA and NCoR in Sertoli cells through a combination of genetic, molecular and pharmacological approaches both in vivo (mutant mice) and in vitro (Sertoli cells in culture).
The postdoctoral position is funded by ANR for a period of 2 years, starting from fall 2018. We are looking for candidates with a PhD degree in molecular and cellular biology, genetics or biochemistry. The candidates will have proven research skills in those fields attested by a track-record of research/academic achievement and publications in internationally recognized peer-reviewed journals. Prior experience with CRISPR/Cas9 technology and immunoprecipitation are essential. Good communication skills (in French and/or English) and team spirit are also indispensable.
If you are interested to apply, please send your CV, a cover letter stating research interests and 2 reference names to Vernet Nadège: firstname.lastname@example.org
IGBMC website: http://www.igbmc.fr/
Team Webpage: http://www.igbmc.fr/research/department/2/team/10/