Inheritance Of Protein Super-Assembly–Based Memory During Adaptation To Oxidative Stress And Aging
Reference : PhD Gilles Charvin
Publication de l'offre : 3 juin 2016
Adaptation to stress is a ubiquitous multi-faceted feature of living organisms, which constantly experience unpredictable changes in their environment. Although mechanisms of adaptation on physiological and genetic time scales have been extensively documented in model species, little is known about adaptation strategies occurring on intermediate time scales. Recent work suggests that the memory of past exposure to a specific environment can be achieved by the formation of protein super-assemblies that serve as the physical support for epigenetic inheritance. ?Our preliminary data indicate that long-term (i.e., intermediate between physiological and genetic) adaptation of yeast to oxidative stress might be mediated by the formation and inheritance of super-assemblies of the Tsa1 peroxiredoxin. Building on these findings, the overall goal of this project is to explore protein super-assembly–based mechanisms of long-term memory of stress and its consequences for both adaptation of the species and the longevity of individuals.
This project combines yeast genetics, microfluidics and live imaging assays to quantitatively characterize the fate of single cells over multi-generation time scales in a changing environment. The applicant will learn standard microfabrication techniques used to monitor yeast cell division, develop image analysis pipelines and build the molecular biology tools required to run the project.
The succesfull PhD applicant will be funded by a grant from the Fondation de la Recherche Médicale (3 years). Candidates with a strong expertise spanning theoretical physics to cell biology will be considered, with a strong emphasis on autonomy, curiosity and self-motivation.
Gilles Charvin ; firstname.lastname@example.org
Date limite de candidature : 31 décembre 2016