4 research departments
750 employees
45 nationalities
55 research teams
16 ERC laureates
260 publications per year
24000 m² lab area

Support us through

Fondation universite de Strasbourg



Tel. : +33 (0)3 88 65 34 40
Tel. : +33 (0)3 88 65 34 45

Key figures

51 research teams
135 researchers
91 post-doctoral fellows
118 PhD students
200 engineers & technicians
22 master students
128 administrators & general services 110 ICS employees

Quick Links

Job opportunities

Epigenetic Regulation Of Gene Expression In Melanoma

Reference : PhD Irwin DAVIDSON

Offer publication : April 5, 2016

Melanoma is a highly aggressive human cancer. MITF (Microphthalmia-associated transcription factor), a bHLH-bZIP familly transcription factor is a master regulator of the physiology of melanoma cells and melanocytes. MITF controls the proliferation and invasion of melanoma cells. To better understand how MITF regulates transcription we have identified several new MITF cofactors, amongst which are the chromatin remodelling complexes PBAF and NURF. We have used integrative functional and genomics approaches in cellulo and genetics in the mouse to define the critical roles of the PBAF subunit BRG1 and the NURF subunit BPTF in melanoma cells and in the melanocyte lineage. The objective of the project will be to better determine the functions of these remodelling complexes in gene regulation in melanoma in vitro and in vivo.
Using melanoma cells, we will investigate the role of each subunit of the PBAF and NURF complexes in control of gene expression, complex integrity and genomic localisation combining knockdown experiments with RNA-seq and ChIP-seq. We have generated recombinant mice with floxed alleles of the genes encoding Brg1 and Bptf such that we can inactive these proteins in a mouse melanoma model. This will allow us to address the role of
these proteins in tumourigenesis in vivo.

- WISHED SKILLS : General expertise in molecular and cellular biology or biochemistry

- EXPERTISES WHICH WILL BE ACQUIRED DURING THE TRAINING : General expertise in molecular and cellular biology, bioinformatics, generation and analyses of genetically modified mice.

Your application

Application Deadline : Dec. 31, 2016

Imprimer Envoyer

Université de Strasbourg

IGBMC - CNRS UMR 7104 - Inserm U 964
1 rue Laurent Fries / BP 10142 / 67404 Illkirch CEDEX / France Tél +33 (0)3 88 65 32 00 / Fax +33 (0)3 88 65 32 01 / directeur.igbmc@igbmc.fr