Quantitative single molecule imaging in living cells and organisms
Dr Christof GEBHARDT
Universität Ulm, Institut für Biophysik, Germany
Friday, July 19th 2019 - 11 a.m.
- Auditorium, IGBMC
Hosted by Nacho MOLINA
In a living organism, numerous inanimate biomolecules and their inherently stochastic interactions are precisely orchestrated and organized in space and time, together enabling the enigma of life. By using single molecule fluorescence microscopy we obtain an exceptionally detailed view on life processes in the natural environment of a live cell or embryo. Tracking individual molecules yields quantitative information such as reaction rates, diffusion coefficients, concentrations and bound fractions and the spatial distribution and stoichiometry of cellular structures. Thus, we obtain information necessary for a deep understanding and modeling of life processes.
I will give a short overview over our imaging methodologies and then focus on recent findings regarding the implications of transcription factor - DNA interaction kinetics on the transcription process, the activation of transcription in zebrafish development and the activity of molecular motors in the cell nucleus.