Regulation of Protein Synthesis by mRNA Structure
Pr Dmitri ERMOLENKO
Department of Biochemistry and Biophysics, University of Rochester, United States
Tuesday, June 25th 2019 - 11 a.m.
- Auditorium, IGBMC
Hosted by Integrated structural Biology, Marat YUSUPOV
During translation elongation, the ribosome moves along mRNA in a codon-by-codon manner. Although the elongating ribosome is an efficient helicase, certain RNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting in both bacteria and eukaryotes. Likewise, basepairing interactions between Shine-Dalgarno (SD) sequences present within open reading frames (ORFs) in mRNAs and the 16S rRNA of bacterial ribosomes were shown to facilitate frameshifting and were suggested to induce ribosome pausing. It remains unclear why mRNAs with certain elements of RNA secondary structure and SD-like sequences pause the ribosome, while others do not. I’m going to present our most recent studies of the mechanisms by which (i) RNA stem-loop structures induce ribosome pausing and (ii) basepairing interactions between the Shine-Dalgarno sequence of mRNA and the 16S rRNA affect ribosome translocation.