Isotope labelling in eukaryotic cells for studying molecular interactions by NMR
Dr Alvar GOSSERT
Institute for Molecular Biology and Biophysics, ETH Zürich, Suisse
jeudi 15 juin 2017 - 14h00
- Salle de conférence E1031, CBI
Invité(e) par Biologie structurale intégrative, Bruno KIEFFER
Molecular interactions are at the heart of biological function and are often targeted in drug discovery. NMR is a very powerful technique to study molecular interactions, be it between two proteins or between a protein and a ligand. The major advantage of NMR is that it can characterize both binding partners at the same time in solution. A pre-requisite for in-depth NMR studies however, are isotopically labelled proteins.
Human proteins – which are most relevant for medicinal applications – can often only be expressed in eukaryotic cells. Until recently no robust and cost effective protocols were available for isotope labelling in eukaryotic cells. We have developed several protocols for labelling in insect cells as well as for amino acid selective labelling in mammalian cells.
A range of applications in drug discovery will be presented which are based on these protocols.